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Proteome Software Inc
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Image Search Results
Journal: International Journal of Bioprinting
Article Title: Bioprinting of Human Neural Tissues Using a Sustainable Marine Tunicate-Derived Bioink for Translational Medicine Applications
doi: 10.18063/ijb.v8i4.604
Figure Lengend Snippet: Standardization of bioink on rheology, printability, cross-linking, and bioprinting of neural tissue constructs. (A) 10% tunicate hydrogel in NSC media is extruded into PBS, and the filament is not dense enough to free flow into the solution, as shown by its upward push when it extrudes into the solution. The filaments broke and fell into the solution when extruded continuously. (B) 10% tunicate gel + 26% Matrigel in NSC media in PBS, the filament is smoothly flowing into the solution. The filaments broke and fell into the solution when extruded continuously for a longer time. (C) 10% tunicate gel in NSC media extruded into the crosslinking solution (250 mM CaCl 2 ) gets cross-linked but lack smooth flow. (D) 10% tunicate gel + 26% Matrigel in NSC media extruded into the cross-linking solution (250 mM CaCl 2 ) shows a seamless extrusion. (E) Filament formation of 10% tunicate hydrogel in NSC media without Matrigel. (F) Filament formation of 10% tunicate gel + 26% Matrigel in NSC media. (G) Droplet formation of 10% tunicate hydrogel in NSC media without Matrigel at the tip of the needle. (H) Droplet formation of 10% tunicate gel +26% Matrigel in NSC media at the tip of the needle. (I) BioCAD design of the tissue construct. (J) Tool path generated using the BioCAD software showing the direction of print head movement. The numbers represent the steps in print head movement. (K) The shear modulus of the hydrogel. (L) Lattice coordinate profile showing structural uniformity of the two layered structure. The upward wave shows the mean struct length and the downward wave shows the total strut thickness. (M) 3D printed lattices in layers ranging from 1 to 10, showing the printability of the hydrogel. (N) Bioprinted tissue constructs in a 24-well plate printed using the well editor software plugin. The dimensions of the bioprinted tissue constructs were 8 mm × 8 mm × 1 mm. Alcian blue dye was used to enhance the visibility of cell-free hydrogel filaments. Neural tissue constructs were printed without the dye.
Article Snippet: Scaffolds were designed and fabricated using RegenHU
Techniques: Construct, Generated, Software, Shear